Phrasal phonology in Copperbelt Bemba

Copperbelt Bemba exhibits several rightward spreading tonal processes which are sensitive to prosodic phrase structure. The rightmost H tone in a word will undergo unbounded spreading if the word is final in a phonological phrase (phi). In an intonational phrase consisting of several single-word phi's, the rightmost H in the first word will spread through all following toneless phi's. From a rule-based perspective, this can only be accounted for by positing mutually feeding iterative rules, as a single H-tone spreading rule cannot account for the long-distance spreading. Rather, a second rule that spreads a H from the final mora of one word onto the initial mora of the following word is required, as a bridge to further unbounded spreading. Three phrase-sensitive OT constraints are proposed to account for H-tone spreading between words. One is of the domain-juncture variety, requiring the specification of two separate prosodic domains

Porin new light onto chromatin and nuclear organization

The capture of associations between chromatin and nuclear pore complexes throws new light on chromosome organization in the nucleus

Encuesta multidisciplinaria en Curré: informe preliminar

The goal of the report that we present in the following has for its objective to make known preliminary results obtained by an Anthropology Team, based ona series of surveys taken in the indigenous community of Curré, cantón Buenos Aires, Costa Rica. The investigators focussed on a series of socio-cultural themes as they were recordedin this Indigenous community: social organization, economic resources, languages, recent revitalization, political elections.El objetivo del informe que presentamos a continuación tiene como finalidad dar a conocer los resultados preliminares obtenidos por un equipo de antropólogos, con base en una serie de encuestas en el pueblo indígena de Curré, cantón de Buenos Aires, Costa Rica. Los investigadores se enfocaron en una serie de temas socioculturales; tales como los encontrados en este pueblo indígena: organización social, recursos económicos, lenguajes, revitalización reciente, elecciones políticas

Views from within a narrative : Evaluating long-term human-robot interaction in a naturalistic environment using open-ended scenarios

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. Date of acceptance: 16/06/2014This article describes the prototyping of human–robot interactions in the University of Hertfordshire (UH) Robot House. Twelve participants took part in a long-term study in which they interacted with robots in the UH Robot House once a week for a period of 10 weeks. A prototyping method using the narrative framing technique allowed participants to engage with the robots in episodic interactions that were framed using narrative to convey the impression of a continuous long-term interaction. The goal was to examine how participants responded to the scenarios and the robots as well as specific robot behaviours, such as agent migration and expressive behaviours. Evaluation of the robots and the scenarios were elicited using several measures, including the standardised System Usability Scale, an ad hoc Scenario Acceptance Scale, as well as single-item Likert scales, open-ended questionnaire items and a debriefing interview. Results suggest that participants felt that the use of this prototyping technique allowed them insight into the use of the robot, and that they accepted the use of the robot within the scenarioPeer reviewe

Usability and acceptability assessment of an empathic virtual agent to prevent major depression

In Human-Computer Interaction, the adaptation of the content and the way of how this content is communicated to the users in interactive sessions is a critical issue to promote the acceptability and usability of any computational system. We present a user-adapted interactive platform to identify and provide an early intervention for symptoms of depression and suicide. In particular, we describe the work performed to assess users' system acceptability and usability. An empathic Virtual Agent is the main interface with the user, and it has been designed to generate the appropriate dialogues and emotions during the interactions according to the detected user's specific needs. This personalization is based on a dynamic user model nurtured with clinical, demographical and behavioural information. The evaluation was performed with 60 participants from the university community. The obtained results were promising, allowing the execution of a further clinical trial. The system's usability score was 75.7%, and the score of the user-adapted content and the emotional responses of the Virtual Agent was 70.9%.The work presented in this manuscript has been partially funded by the Conselleria de Sanidad of Generalitat Valenciana, in the research project entitled 'Sistema computacional de ayuda a la prevencion de episodios de depresion y suicidio - PREVENDEP'. We thank the company Faceshift (www.faceshift.com) for providing their software to perform facial motion capture in order to develop the talking head that represent our empathic virtual agent.Bresó Guardado, A.; Martinez-Miranda, J.; Botella Arbona, C.; Baños Rivera, RM.; García Gómez, JM. (2016). Usability and acceptability assessment of an empathic virtual agent to prevent major depression. Expert Systems. 33(4):297-312. doi:10.1111/exsy.12151S29731233

DNA Damage, Homology-Directed Repair, and DNA Methylation

To explore the link between DNA damage and gene silencing, we induced a DNA double-strand break in the genome of Hela or mouse embryonic stem (ES) cells using I-SceI restriction endonuclease. The I-SceI site lies within one copy of two inactivated tandem repeated green fluorescent protein (GFP) genes (DR-GFP). A total of 2%–4% of the cells generated a functional GFP by homology-directed repair (HR) and gene conversion. However, ~50% of these recombinants expressed GFP poorly. Silencing was rapid and associated with HR and DNA methylation of the recombinant gene, since it was prevented in Hela cells by 5-aza-2′-deoxycytidine. ES cells deficient in DNA methyl transferase 1 yielded as many recombinants as wild-type cells, but most of these recombinants expressed GFP robustly. Half of the HR DNA molecules were de novo methylated, principally downstream to the double-strand break, and half were undermethylated relative to the uncut DNA. Methylation of the repaired gene was independent of the methylation status of the converting template. The methylation pattern of recombinant molecules derived from pools of cells carrying DR-GFP at different loci, or from an individual clone carrying DR-GFP at a single locus, was comparable. ClustalW analysis of the sequenced GFP molecules in Hela and ES cells distinguished recombinant and nonrecombinant DNA solely on the basis of their methylation profile and indicated that HR superimposed novel methylation profiles on top of the old patterns. Chromatin immunoprecipitation and RNA analysis revealed that DNA methyl transferase 1 was bound specifically to HR GFP DNA and that methylation of the repaired segment contributed to the silencing of GFP expression. Taken together, our data support a mechanistic link between HR and DNA methylation and suggest that DNA methylation in eukaryotes marks homologous recombined segments

Interphase chromosome positioning in in vitro porcine cells and ex vivo porcine tissues

Copyright @ 2012 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and 85 reproduction in any medium, provided the original author and source are credited. The article was made available through the Brunel University Open Access Publishing Fund.BACKGROUND: In interphase nuclei of a wide range of species chromosomes are organised into their own specific locations termed territories. These chromosome territories are non-randomly positioned in nuclei which is believed to be related to a spatial aspect of regulatory control over gene expression. In this study we have adopted the pig as a model in which to study interphase chromosome positioning and follows on from other studies from our group of using pig cells and tissues to study interphase genome re-positioning during differentiation. The pig is an important model organism both economically and as a closely related species to study human disease models. This is why great efforts have been made to accomplish the full genome sequence in the last decade. RESULTS: This study has positioned most of the porcine chromosomes in in vitro cultured adult and embryonic fibroblasts, early passage stromal derived mesenchymal stem cells and lymphocytes. The study is further expanded to position four chromosomes in ex vivo tissue derived from pig kidney, lung and brain. CONCLUSIONS: It was concluded that porcine chromosomes are also non-randomly positioned within interphase nuclei with few major differences in chromosome position in interphase nuclei between different cell and tissue types. There were also no differences between preferred nuclear location of chromosomes in in vitro cultured cells as compared to cells in tissue sections. Using a number of analyses to ascertain by what criteria porcine chromosomes were positioned in interphase nuclei; we found a correlation with DNA content.This study is partly supported by Sygen International PLC

relating conversational expressiveness to social presence and accpetance of an assistive social robot

Exploring the relationship between social presence, conversational expressiveness, and robot acceptance, we set up an experiment with a robot in an eldercare institution, comparing a more and less social condition. Participants showed more expressiveness with a more social agent and a higher score on expressiveness correlated with higher scores on social presence. Furthermore, scores on social presence correlated with the scores on the intention to use the system in the near future. However, we found no correlation between conversational expressiveness and robot acceptance